Proteins were incubated with CAA (4.5 mM) in the dark and resolved on SDS-PAGE. In-gel digestion using trypsin followed prior to subjection of the peptides to LC−MS/MS analysis. Differential protein ...
RNA was extracted with TRIzol reagent (Invitrogen) and purified using the Direct-zol RNA kit (Zymo Research, R2052) from mES cells cultured in LIF or collected over a 7-day time course after LIF ...
Customer stories Events & webinars Ebooks & reports Business insights GitHub Skills ...
Customer stories Events & webinars Ebooks & reports Business insights GitHub Skills ...
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